| Guest star | Role(s) | Episode(s) | Notes |
|---|
| Julie Adams | Nurse Mary Simpson | "The County Nurse" (2.24) |
| Jack Albertson | Bradford J.
Taylor | "Aunt Bee's Cousin" (8.13) | Later known for Chico and the Man, Willy Wonka & the Chocolate Factory, and The Fox and the Hound |
| Norman Alden | Tom Strongbow
Hank | "The Battle of Mayberry" (6.29)
"Howard, the Bowler" (8.02) | Known for voice work including as Aquaman in Super Friends and The All-New Super Friends Hour |
| Leon Ames | Mr.
Hampton | "The Senior Play" (7.09) | Known for the role of Col. Gordon Kirkwood on Mister Ed |
| David Alan Bailey | Fred Simpson | "Opie's First Love" (8.01) | Played the recurring role of Opie's friend Trey Bowden during season 4 |
| Jack Bannon | Announcer | "The Mayberry Chef" (8.17) | Later known for portraying Art Donovan on Lou Grant |
| James Best | Jim Lindsey | "The Guitar Player" (1.03)
"The Guitar Player Returns" (1.31) | Best known fo
|